Pengaruh Pemberian Minyak Ikan Lemuru Terhadap Kadar Glukosa Darah Puasa Pada Tikus Wistar Hiperglikemia

Authors

  • Dea Amanda Caressa Program Studi Ilmu Kesehatan Masyarakat, Fakultas Kesehatan Masyarakat, Universitas Airlangga, Surabaya
  • Annis Catur Adi Program Studi Ilmu Kesehatan Masyarakat, Fakultas Kesehatan Masyarakat, Universitas Airlangga, Surabaya
  • Sri Adiningsih Program Studi Ilmu Kesehatan Masyarakat, Fakultas Kesehatan Masyarakat, Universitas Airlangga, Surabaya
  • Septyaningrum Putri Purwoto Program Studi Pendidikan Olahraga, STKIP PGRI Bangkalan, Bangkalan
  • Adi Pranoto Program Studi Ilmu Kedokteran, Fakultas Kedokteran, Universitas Airlangga, Surabaya

DOI:

https://doi.org/10.21776./ub.ijhn.2022.009.01.3

Keywords:

Minyak ikan lemuru, omega-3, hiperglikemia, kadar glukosa darah puasa

Abstract

Penelitian ini bertujuan menganalisis efek pemberian minyak ikan lemuru terhadap penurunan kadar glukosa darah puasa pada tikus dengan hiperglikemia. Penelitian ini merupakan experimental laboratories dengan racangan The Randomized Post Test Only Control Group Design. Sampel penelitian ini adalah 25 ekor tikus jenis Rattus Norvegicus Strain Wistar jantan, usia 2-3 bulan yang dibagi menjadi lima kelompok. Teknik analisis data menggunakan uji one-way ANOVA dan Tukey HSD. Pada uji one-way ANOVA diadaptkan nilai P (0,001) < 0.05. Hasil Tukey HSD didapatkan rata-rata kadar GDP antara K0 dengan K1 (P < 0,005), P1 dengan K1 (P < 0,005), P2 dengan K1 (P < 0,005), P3 dengan K1 (P < 0,005), sedangkan K0 dengan P1, K0 dengan P2, K0 dengan P3, P1 dengan P2, P1 dengan P3, dan P2 dengan P3 tidak menunjukkan perbedaan signifikan (P > 0,05). Berdasarkan hasil penelitian disimpulkan bahwa terdapat perbedaan pengaruh yang signifikan pemberian minyak ikan lemuru (sardinella lemuru) terhadap kadar glukosa darah puasa. Terdapat perbedaan yang signifikan antara K0 dengan K1, P1 dengan K1, P2 dengan K1, P3 dengan K1, sedangkan K0 dengan P1, K0 dengan P2, K0 dengan P3, P1 dengan P2, P1 dengan P3, dan P2 dengan P3 tidak menunjukkan perbedaan yang signifikan.

References

  • Akrom, Harjanti, & Armansyah. (2014). Efek Hipoglikemik Ekstrak Etanol Umbi Ketela Rambat(Ipomoea batatas P) (EEUKR) pada Mencit Swiss yang DiinduksiAloksan. Pharmaciana, 4(1), 65–76.
  • American Diabetes Association. (2017). Standards of Medical Care in Diabetes. Diabetes Care. 38(1), S1–S93.
  • American Diabetes Association (ADA). (2018). Standards of medical care in diabetes. Diabetes Care. 37(suppl 1): S1.
  • Calder, P. C. (2012). Long-chain fatty acids and inflammation. Proceedings of the Nutrition Society, 71(2), 284–289. https://doi.org/10.1017/S0029665112000067
  • Chapkin, R. S., McMurray, D. N., Davidson, L. A., Patil, B. S., Fan, Y.-Y., & Lupton, J. R. (2011). Bioactive dietary long-chain fatty acids: Emerging mechanisms of action. British Journal of Nutrition, 100(6), 1152–1157. https://doi.org/10.1017/S0007114508992576
  • Cockbain, A. J., Toogood, G. J., & Hull, M. A. (2012). Omega-3 polyunsaturated fatty acids for the treatment and prevention of colorectal cancer. Gut, 61(1), 135–149. https://doi.org/10.1136/gut.2010.233718
  • De Caterina, R., Madonna, R., Bertolotto, A., & Schmidt, E. B. (2007). n-3 Fatty Acids in the Treatment of Diabetic Patients: Biological rationale and clinical data. Diabetes Care, 30(4), 1012–1026. https://doi.org/10.2337/dc06-1332
  • Drugs.com. (2015). Glimepiride Side Effects. 2015. (Http://www.drugs.com/sfx/glimepirideside—Effects.html. Diakses 5 februari 2021).
  • Engström, K., Saldeen, A.-S., Yang, B., Mehta, J. L., & Saldeen, T. (2019). Effect of fish oils containing different amounts of EPA, DHA, and antioxidants on plasma and brain fatty acids and brain nitric oxide synthase activity in rats. Upsala Journal of Medical Sciences, 114(4), 206–213. https://doi.org/10.3109/03009730903268958
  • Estiasih, & Ahmadi. (2012). 2012. Pembuatan trigliserida kaya asam lemak ω-3 dari minyak hasil samping pengalengan ikan lemuru (Sardinella longiceps). Jurnal Teknologi Pertanian, 5(3), 116–128.
  • Farsi, D. (2016). Prevalence of obesity in elementary school children and its association with dental caries. Saudi Med J. 12, 37.
  • Farsi, P. F., Djazayery, A., Eshraghian, M. R., Koohdani, F., Saboor-Yaraghi, A. A., Derakhshanian, H., Zarei, M., Javanbakht, M. H., & Djalali, M. (2014). Effects of supplementation with omega-3 on insulin sensitivity and non-esterified free fatty acid (NEFA) in type 2 diabetic patients. Arquivos Brasileiros de Endocrinologia & Metabologia, 58(4), 335–340. https://doi.org/10.1590/0004-2730000002861
  • Flachs, P., Rossmeisl, M., & Kopecky, J. (2014). The Effect of n-3 Fatty Acids on Glucose Homeostasis and Insulin Sensitivity. Physiological Research, S93–S118. https://doi.org/10.33549/physiolres.932715
  • Fuentes, N. R., Kim, E., Fan, Y.-Y., & Chapkin, R. S. (2018). Omega-3 fatty acids, membrane remodeling and cancer prevention. Molecular Aspects of Medicine, 64, 79–91. https://doi.org/10.1016/j.mam.2018.04.001
  • Gao, H., Geng, T., Huang, T., & Zhao, Q. (2017). Fish oil supplementation and insulin sensitivity: A systematic review and meta-analysis. Lipids in Health and Disease, 16(1), 131. https://doi.org/10.1186/s12944-017-0528-0
  • Ghafoorunissa. (2018). Role of trans fatty acids in health and challenges to their reduction in Indian foods. Asia Pac J Clin Nutr, 17(1), 212–215.
  • Ghidurus, Turtoi, Boskou, & Niculita. (2015). Nutritional and Health Aspects Related to Frying (I). Romanian Biotechnological Letters, 15(5), 121–123.
  • Girón, M. D., Salto, R., Hortelano, P., Periago, J. L., Vargas, A. M., & Suárez, M. D. (1999). Increased diaphragm expression of GLUT4 in control and streptozotocin-diabetic rats by fish oil-supplemented diets. Lipids, 34(8), 801–807. https://doi.org/10.1007/s11745-999-0426-0
  • Hussein, J., Mostafa, E., El-Waseef, M., El-Khayat, Z., Badawy, E., & Medhat, D. (2011). Effect of Omega-3 Fatty Acids on Erythrocyte Membrane in Diabetic Rats. Macedonian Journal of Medical Sciences, 4(3), 234–239. https://doi.org/10.3889/MJMS.1857-5773.2011.0180
  • Ibrahim, Suptijah, & Yogaswara. (2015). Karakterisasi Minyak Ikan Dari Hasil Samping Industri Penepungan Ikan Lemuru (Sardinella Lemuru) Dengan Metode Pemurnian Alkali. Dinamika Maritim, 1, 1–7.
  • IDF. (2017). International DIabetes Federation Diabetes Atlas 8th ed2017.
  • Itsiopoulos, C., Marx, W., Mayr, H. L., Tatucu-Babet, O. A., Dash, S. R., George, E. S., Trakman, G. L., Kelly, J. T., Thomas, C. J., & Brazionis, L. (2018). The role of omega-3 polyunsaturated fatty acid supplementation in the management of type 2 diabetes mellitus: A narrative review. Journal of Nutrition & Intermediary Metabolism, 14, 42–51. https://doi.org/10.1016/j.jnim.2018.02.002
  • Iwase, Y., Kamei, N., & Takeda-Morishita, M. (2015). Antidiabetic Effects of Omega-3 Polyunsaturated Fatty Acids: From Mechanism to Therapeutic Possibilities. Pharmacology & Pharmacy, 06(03), 190–200. https://doi.org/10.4236/pp.2015.63020
  • Jiang, Zhu, & Mcginley. (2012). Identification of a molecular signature underlying inhibition of mammary carcinoma growth by dietary n-3 fatty acids. Cancer Res, 72, fatty acids. Cancer Res. 2012, 72, 3795–3806.
  • Karpe, F., Dickmann, J. R., & Frayn, K. N. (2011). Fatty Acids, Obesity, and Insulin Resistance: Time for a Reevaluation. Diabetes, 60(10), 2441–2449. https://doi.org/10.2337/db11-0425
  • Keapai, W., Apichai, S., Amornlerdpison, D., & Lailerd, N. (2016). Evaluation of fish oil-rich in MUFAs for anti-diabetic and anti-inflammation potential in experimental type 2 diabetic rats. The Korean Journal of Physiology & Pharmacology, 20(6), 581. https://doi.org/10.4196/kjpp.2016.20.6.581
  • Kris-Etherton, P. M., Harris, W. S., & Appel, L. J. (2002). Fish Consumption, Fish Oil, Omega-3 Fatty Acids, and Cardiovascular Disease. Circulation, 106(21), 2747–2757. https://doi.org/10.1161/01.CIR.0000038493.65177.94
  • Kromhout, D., Geleijnse, J. M., de Goede, J., Oude Griep, L. M., Mulder, B. J. M., de Boer, M.-J., Deckers, J. W., Boersma, E., Zock, P. L., & Giltay, E. J. (2011). N-3 Fatty Acids, Ventricular Arrhythmia-Related Events, and Fatal Myocardial Infarction in Postmyocardial Infarction Patients With Diabetes. Diabetes Care, 34(12), 2515–2520. https://doi.org/10.2337/dc11-0896
  • Kumar, S., & Satish, C. (2014). Insulin Resistance. Asian Pacific Journal of Health Sciences, 1(4s), 71–78.
  • Larasati, R., Wirjatmadi, B., & Merryana, A. (2016). Pengaruh Pemberian Trans Fatty Acid (Tfa) Dari Margarin Dan Minyak Kelapa Sawit Yang Dipanaskan Berulang Terhadap Kadar Glukosa Darah Puasa Pada Tikus Wistar. The Indonesian Journal of Public Health, II(1), 69–77.
  • Ley, S. H., Hamdy, O., Mohan, V., & Hu, F. B. (2014). Prevention and management of type 2 diabetes: Dietary components and nutritional strategies. The Lancet, 383(9933), 1999–2007. https://doi.org/10.1016/S0140-6736(14)60613-9
  • Lorente, C., Costa, A., Carretero, S., Zabala, M., & Martinez, A. (2013). Role of omega-3 fatty acids in obesity, metabolic syndrome, and cardiovascular diseases: A review of the evidence. Journal of Physiology and Biochemistry volume, 69, 633–651.
  • Mahmood Freije, A., & Nedham Awadh, M. (2011). Fatty acid compositions of Turbo coronatus Gmelin 1791. British Food Journal, 112(10), 1049–1062. https://doi.org/10.1108/00070701011080195
  • Manduapessy. (2017). Profil Asam Lemak Ikan Layang Segar (Decapterus macrosama). Majalah Biam Vol 13 (1): 42-46Winarno, F.G. (2002). Kimia Pangan dan Gizi. Jakarta: PT. Gramedia Pustaka Utama.
  • Martorell, M., Capó, X., Sureda, A., Batle, J. M., Llompart, I., Argelich, E., Tur, J. A., & Pons, A. (2014). Effect of DHA on plasma fatty acid availability and oxidative stress during training season and football exercise. Food & Function, 5(8), 1920. https://doi.org/10.1039/C4FO00229F
  • Minarul, I. (2014). Effects of Conjugated Linoleic Acid and Hilsha Fish Oil (Tenualosa Ilisha) on Alloxan Induced Diabetes Mellitus in Sprague Dawley Rats. Journal of Diabetes & Metabolism, 05(08). https://doi.org/10.4172/2155-6156.1000412
  • Ogbru, Williams, & Marks. (2015). Insulin: Drug Facts, Side Effects and Dosing. (Http://www.medicinenet.com/insulin/article.htm. Diakses 5 februari 2021).
  • Oh, D. Y., Talukdar, S., Bae, E. J., Imamura, T., Morinaga, H., Fan, W., Li, P., Lu, W. J., Watkins, S. M., & Olefsky, J. M. (2010). GPR120 Is an Omega-3 Fatty Acid Receptor Mediating Potent Anti-inflammatory and Insulin-Sensitizing Effects. Cell, 142(5), 687–698. https://doi.org/10.1016/j.cell.2010.07.041
  • Pingali, U., Nutalapati, C., & Illendulla, V. S. (2020). Evaluation of the Effect of Fish Oil Alone and in Combination with a Proprietary Chromium Complex on Endothelial Dysfunction, Systemic Inflammation and Lipid Profile in Type 2 Diabetes Mellitus – A Randomized, Double-Blind, Placebo-Controlled Clinical Study. Diabetes, Metabolic Syndrome and Obesity: Targets and Therapy, Volume 13, 31–42. https://doi.org/10.2147/DMSO.S220046
  • Raghu, B., & Venkatesan, P. (2018). Effect of n-3 fatty acid supplementation on blood glucose, lipid profile and cytokines in humans: A pilot study. Indian Journal of Clinical Biochemistry, 23(1), 85–88. https://doi.org/10.1007/s12291-008-0020-8
  • Rossi, A. S., Lombardo, Y. B., Lacorte, J.-M., Chicco, A. G., Rouault, C., Slama, G., & Rizkalla, S. W. (2015). Dietary fish oil positively regulates plasma leptin and adiponectin levels in sucrose-fed, insulin-resistant rats. American Journal of Physiology-Regulatory, Integrative and Comparative Physiology, 289(2), R486–R494. https://doi.org/10.1152/ajpregu.00846.2004
  • Rubio-Rodríguez, N., Beltrán, S., Jaime, I., de Diego, S. M., Sanz, M. T., & Carballido, J. R. (2010). Production of omega-3 polyunsaturated fatty acid concentrates: A review. Innovative Food Science & Emerging Technologies, 11(1), 1–12. https://doi.org/10.1016/j.ifset.2009.10.006
  • Sarker, S. (2020). By-products of fish-oil refinery as potential substrates for biogas production in Norway: A preliminary study. Results in Engineering, 6, 100137. https://doi.org/10.1016/j.rineng.2020.100137
  • Sharma, Katyal, Grewal, Behera, & Budhiraja. (2012). Effect of Antioxidants Such as βCarotene, Vitamin C and Vitamin E on Oxidative Stress, Thermal Hyperalgesia and Cold Allodyna in Streptozotocin Induced Diabetic Rats. The International Jurnal of Pharmacology, 1531–2976.
  • Suarsana, I. N., Utama, I. H., Agung, I. G., & Suartini, A. (2011). Pengaruh Hiperglikemia dan Vitamin E pada Kadar Malonaldehida dan Enzim Antioksidan Intrasel Jaringan Pankreas Tikus. Majalah Kedokteran Bandung, 43(2), 72–76. https://doi.org/10.15395/mkb.v43n2.46
  • Sutama, Achmad, & Rachma. (2017). Kejadian Efek Samping Potensial Terapi Obat Anti Diabetes Pasien Diabetes Melitus Berdasarkan Algoritma Naranjo. Pharmaceutical Journal Of Indonesia, 2(2), 45–50.
  • Thadeus, M. (2015). Dampak Konsumsi Minyak Jelantah Terhadap Kerusakan Oksidatif DNA (Kajian Aspek: Biologi Molekuler dan Imunologi)’, Yogyakarta.
  • Ting, Szeto, Chan, Ma, & Chow. (2006). Risk factors of vitamin B(12) deficiency in patients receiving metformin. Arch Intern Med, 166(18), 1975–1979.
  • Toupchian, O., Sotoudeh, G., Mansoori, A., Nasli-Esfahani, E., Djalali, M., Keshavarz, S. A., & Koohdani, F. (2016). Effects of DHA-enriched fish oil on monocyte/macrophage activation marker sCD163, asymmetric dimethyl arginine, and insulin resistance in type 2 diabetic patients. Journal of Clinical Lipidology, 10(4), 798–807. https://doi.org/10.1016/j.jacl.2016.02.013
  • Ulven, S. M., Kirkhus, B., Lamglait, A., Basu, S., Elind, E., Haider, T., Berge, K., Vik, H., & Pedersen, J. I. (2011). Metabolic Effects of Krill Oil are Essentially Similar to Those of Fish Oil but at Lower Dose of EPA and DHA, in Healthy Volunteers. Lipids, 46(1), 37–46. https://doi.org/10.1007/s11745-010-3490-4
  • Vaughan, R. A., Garcia-Smith, R., Bisoffi, M., Conn, C. A., & Trujillo, K. A. (2012). Conjugated linoleic acid or omega 3 fatty acids increase mitochondrial biosynthesis and metabolism in skeletal muscle cells. Lipids in Health and Disease, 11(1), 142. https://doi.org/10.1186/1476-511X-11-142
  • Wang, L. Z., Cheung, C. Y., Tapp, R. J., Hamzah, H., Tan, G., Ting, D., Lamoureux, E., & Wong, T. Y. (2017). Availability and variability in guidelines on diabetic retinopathy screening in Asian countries. British Journal of Ophthalmology, 101(10), 1352–1360. https://doi.org/10.1136/bjophthalmol-2016-310002
  • Webel, A. R., & Higgins, P. A. (2012). The Relationship Between Social Roles and Self-Management Behavior in Women Living with HIV/AIDS. Women’s Health Issues, 22(1), e27–e33. https://doi.org/10.1016/j.whi.2011.05.010
  • WHO. (2015). Diabetes Programme about World Diabetes Day 2015.
  • WHO. (2019). Classification of Diabetes Mellitus 2019.
  • Winarno. (2004). Kimia Pangan dan Gizi. Jakarta (ID): Gramedia Pustaka Utama.
  • Xin, W., Wei, W., & Li, X. (2012). Effect of Fish Oil Supplementation on Fasting Vascular Endothelial Function in Humans: A Meta-Analysis of Randomized Controlled Trials. PLoS ONE, 7(9), e46028. https://doi.org/10.1371/journal.pone.0046028.
  • Yao, F., Zhang, M., & Chen, L. (2016). 5’-Monophosphate-activated protein kinase (AMPK) improves autophagic activity in diabetes and diabetic complications. Acta Pharmaceutica Sinica B, 6(1), 20–25. https://doi.org/10.1016/j.apsb.2015.07.009.

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2022-07-30

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